| 徐婷婷，熊玥，张婷，严士海，吴坚，刘沈林，刘史佳，张其德.基于液质联用技术的健脾养正消癥方治疗胃癌小鼠血清代谢组学分析[J].南京中医药大学学报(社会科学版),2021,37(2):237-243. |
| 基于液质联用技术的健脾养正消癥方治疗胃癌小鼠血清代谢组学分析 |
| Analysis of Serum Metabolome in Mice with Gastric Cancer Treated with JPYZXZ Prescription Based on Liquid Chromatography-Mass spectrometry |
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| DOI： |
| 中文关键词: 关键词：胃癌 健脾养正消癥方 代谢组学 超高效液相色谱-质谱 差异代谢物 |
| 英文关键词: gastric cancer JPYZXZ prescription metabolomics ultra performance liquid chromatography-mass spectrometry differential metabolites |
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| 中文摘要: |
| 目的 通过代谢组学方法，观察给予健脾养正消癥方的前后胃癌小鼠血清代谢产物变化规律，寻找其与治疗胃癌疗效相关特征分子，探讨健脾养正消癥方在调控胃癌内源性机制方面作用。方法 建立人胃癌裸鼠皮下移植瘤模型，并随机分为模型组，健脾养正消癥方低剂量组和健脾养正消癥方高剂量组。采用超高效液相色谱串联四级杆飞行时间质谱（UPLC-Q-TOF/MS）技术，对各组小鼠血清样本进行分析，结合主成分分析法（PCA）和正交偏最小二乘法判别分析（OPLS-DA）来筛选差异性代谢物，利用MetaboAnalyst网站分析相关代谢通路。结果 健脾养正消癥方高、低剂量组小鼠瘤体平均瘤质量明显小于模型组（P<\i><0.05）。代谢组学结果经分析，模型组、低剂量组和高剂量组血清样本能够得到很好的区分，健脾养正消癥方给药后胃癌小鼠的内源性代谢物水平发生不同程度的回调，并初步鉴定出5个差异代谢物和4条相关代谢通路。结论 健脾养正消癥方能够明显抑制小鼠肿瘤的生长，其作用机制可能与花生四烯酸水平的升高以及其激活的α-亚麻酸和亚油酸代谢和花生四烯酸代谢通路有关。 |
| 英文摘要: |
| OBJECTIVE To observe the changes of serum metabolites in mice with gastric cancer before and after giving JPYZXZ prescription by metabolomics， and to find the characteristic molecules related to the efficacy of the prescription in the treatment of gastric cancer， and to explore the role of JPYZXZ prescription in regulating the local microenvironment and endogenous mechanism of gastric cancer. METHODS A subcutaneous transplantation tumor model of human gastric cancer in nude mice was established and randomly divided into a model group， a low-dose group of JPYZXZ prescription， and a high-dose group of JPYZXZ prescription. The serum samples of each group were detected by ultra performance liquid chromatography-mass spectrometry (UPLC-Q-TOF/MS)， the differential metabolites were screened by principal components analysis (PCA) and orthogonal partial least squares- discriminant analysis (OPLS-DA)，and MetaboAnalyst was used to analyze the relevant metabolic pathways. RESULTS The mean tumor weight of mice in the high and low dose groups was significantly smaller than that of the model group (P<\i><0.05). The results of metabolomics showed that the serum samples of the model group， the low-dose group， and the high-dose group could be well distinguished， and the levels of endogenous metabolites in the mice with gastric cancer were varying degrees of callbacks after the administration of JPYZXZ prescription， and 5 different metabolites and 4 related metabolic pathways were identified preliminarily. CONCLUSION JPYZXZ prescription can significantly inhibit the growth of tumors in mice， and its mechanism of action may be related to the increase of arachidonic acid level and the activation of α-linolenic acid and linoleic acid metabolism and arachidonic acid metabolic pathway. |
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