| 王珏，郭钰昕，姚毅，张剑，李津，朱育凤，刘史佳.基于HPLC指纹图谱的半夏及其伪品鉴别研究[J].南京中医药大学学报(社会科学版),2020,36(2):267-272. |
| 基于HPLC指纹图谱的半夏及其伪品鉴别研究 |
| Identification of Pinellia Ternata<\i> and Its Counterfeits Based on HPLC Fingerprint Analysis |
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| DOI： |
| 中文关键词: 关键词：半夏 指纹图谱 伪品 鉴别 质量控制 |
| 英文关键词: Pinellia ternata<\i> fingerprints counterfeit identification quality control |
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| 摘要点击次数: 801 |
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| 中文摘要: |
| 目的 建立高效液相色谱法（HPLC）对半夏及其伪品进行指纹图谱分析，以鉴定半夏的真伪。方法 用SB-Aq色谱柱（4.6 mm×250 mm，5 μm），以0.2%磷酸二氢铵溶液-甲醇为流动相进行梯度洗脱，进样量20 μL，流速0.8 mL/min，柱温30 ℃，采用双波长检测。结果 生半夏共有10个共有峰，通过对照品比对指认了其中5个共有峰；伪品半夏中同时含有8种有效成分；随机选取部分生半夏与伪品半夏样品建立叠加指纹图谱得出：虎掌南星于34~38 min出现2个特征峰；姜水半夏在32~34 min出现2个特征峰；而水半夏出峰整体峰高较低，峰面积偏小。结论 建立的指纹图谱特征性强，能明显区分半夏与其伪品水半夏及虎掌南星，可用于半夏的鉴别和质量控制。 |
| 英文摘要: |
| OBJECTIVE To develop a high performance liquid chromatography （HPLC） method for the fingerprint analysis of Pinellia ternata <\i>and its authenticity identification. METHODS HPLC analysis was performed on a SB-Aq column（4.6 mm×250 mm， 5 μm） with the mixture of 0.2% ammonium dihydrogen phosphate solution ( pH=3.0) (A) and methanol (B) as mobile phase in gradient mode. The column temperature was set at 30 ℃ and dual wavelength detection was used. RESULTS The HPLC fingerprint of Pinellia ternata<\i>，showing 10 characteristic peaks，was established from raw Pinellia ternata<\i>， and 5 were identified by standard comparison， and 8 common constituents were identified in fake Pinellia ternata <\i>samples. An overlay fingerprint were established with samples randomly selected： From 34 to 38 min，there were two characteristic peaks in the Pedate Rhizome Pinellia<\i>， and two appeared at 32 to 34 min in the Rhizoma Typhonii Flagelliformis <\i>prepared with ginger juice，while the peak height in the raw Typhonii flagelliformis Rhizoma <\i>was lower and the peak area was smaller. CONCLUSION The fingerprint of Pinellia ternata <\i>is significantly different from its confused herbs， which can be applied to the identification and quality control of Pinellia ternata. |
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