| 颜媛媛，朱宇超，张焱，嵇晶，王琪，张晓云，何瑞欣，徐雅蝶，陈仁寿，程建明.经典名方清上蠲痛汤指纹图谱研究[J].南京中医药大学学报(社会科学版),2020,36(2):259-266. |
| 经典名方清上蠲痛汤指纹图谱研究 |
| Study on Fingerprint in TCM Classical Prescription of Qingshang Juantong Decoction |
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| DOI： |
| 中文关键词: 关键词：清上蠲痛汤 HPLC 指纹图谱 HPLC-Q-TOF/MS；化学成分 |
| 英文关键词: Qingshang Juantong Decoction HPLC fingerprint HPLC-Q-TOF/MS chemical constituents |
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| 中文摘要: |
| 目的 建立清上蠲痛汤水煎液指纹图谱，并对其共有峰进行分析和指认。方法 采用HPLC-UV建立清上蠲痛汤复方指纹图谱，使用Agilent Poroshell 120 EC-C18柱（4.6 mm×150 mm，4 μm），以甲醇-乙腈-0.25%冰乙酸为流动相，进行梯度洗脱，流速1.0 mL/min，柱温35 ℃，进样量5 μL；检测波长选择355 nm；采用HPLC-Q-TOF/MS进行定性分析，再进行对照品比对验证。结果 方法学考察表明，所用色谱方法符合指纹图谱技术定性研究要求。标定34个色谱峰，并指认其中14个峰，分别为绿原酸、阿魏酸、木犀草苷、紫花前胡苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、蔓荆子黄素、藁本内酯、蛇床子素、羌活醇、异欧前胡素、苍术素。经《中药色谱指纹图谱相似度评价系统》(2012版)对10批清上蠲痛汤水煎液进行考察，相似度均在0.97以上。结论 该法灵敏度高，稳定性和准确性良好，体现了清上蠲痛汤组分的整体特征，为清上蠲痛汤水煎液质量标准的建立提供了可靠的依据。 |
| 英文摘要: |
| OBJECTIVE To establish a fingerprint of Qingshang Juantong Decoction (QSD)， analyze and identify the common peaks. METHODS HPLC-UV was used to establish fingerprint of QSD; Agilent Poroshell 120 EC-C18 column (150 mm×4.6 mm， 4 μm) was used， eluting with a gradient of methanol-acetonitrile-0.25% glacial acetic acid. Flow rate was 1.0 mL/min， the column temperature was 35 ℃， the injection volume was 5 μL， and the detection wavelength was 355 nm. HPLC-Q-TOF/MS was used for qualitative analysis and the comparison was conducted for verification. RESULTS The methodological study showed that the chromatographic method established met the qualitative research requirements of fingerprinting technology. A total of 34 peaks were shared， and 14 peaks were identified， namely chlorogenic acid， ferulic acid， cynaroside， nodakenin， baicalin， wogonoside， baicalein， wogonin， vitexicarpin， ligustilide， osthole， notopterol， lisoimperatorin， atractylodin. According to the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" (2012 edition)， 10 batches of QSD were investigated， and the similarity was above 0.97. CONCLUSION This method shows high sensitivity， stability and accuracy， which reflects the overall characteristics of Qingshang Juantong Decoction and provides a reliable scientific basis for the establishment of Qingshang Juantong Decoction. |
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