| 吕兴，吕高虹，叶冠.香参壳方对人胃癌细胞上皮间质转化及转移的影响[J].南京中医药大学学报(社会科学版),2019,35(6):687-690. |
| 香参壳方对人胃癌细胞上皮间质转化及转移的影响 |
| Protective Effect of Xiangshenke Formula on the Epithelial Mesenchymal Transformation and Metastasis of Human Gastric Carcinoma Cells |
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| 中文关键词: 关键词：香参壳方 人胃癌细胞 SGC-7901 上皮间质转化 GSK3β/β-Catenin信号通路 |
| 英文关键词: Xiangshenke formula human gastric carcinoma cells SGC-7901 epithelial-mesenchymal transition GSK3β/β-Catenin signaling pathway |
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| 中文摘要: |
| 目的 考察香参壳方调节人胃癌细胞SGC-7901上皮间质转化(EMT)及转移的作用机制。方法 体外培养SGC-7901细胞，随后加入不同浓度的香参壳方含药血清进行干预。划痕实验及Transwell小室法观察SGC-7901细胞的迁移与侵袭能力，MTT法考察SGC-7901细胞的增殖能力，Western blot法检测细胞中N-cadherin、Vimentin、E-cadherin、p-GSK3β/GSK3β、β-Catenin表达水平。结果 香参壳方可有效抑制SGC-7901细胞的迁移及侵袭能力，抑制其增殖，同时下调细胞中N-cadherin、Vimentin、p-GSK3β/GSK3β及β-Catenin的表达，上调E-cadherin的表达。结论 香参壳方主要通过介导GSK3β/β-Catenin信号通路抑制胃癌细胞的EMT，降低弱细胞迁移与侵袭能力，从而发挥其胃癌术后的辅助治疗作用。 |
| 英文摘要: |
| OBJECTIVE To investigate the mechanism of Xiangshenke formula in regulating the proliferation， migration and EMT of human gastric carcinoma cells SGC-7901. METHODS SGC-7901 cells were cultured in vitro<\i>， and then different concentrations of Xiangshenke formula containing serum were added into the cells. Wound healing test and transwell chamber assay were used to observe the SGC-7901 cell migration and invasion. MTT assay was used to investigate the SGC-7901 cell proliferation. Western blot was used to detect the expression levels of N-cadherin， Vimentin， E-cadherin， p-GSK3β/GSK3β and β-Catenin. RESULTS Xiangshenke formula effectively inhibited the migration and invasion of SGC-7901 cells， suppressed the cell proliferation， down-regulated the expression of N-cadherin， Vimentin， p-GSK3β/GSK3β and β-Catenin， and up-regulated the expression of E-cadherin. CONCLUSION Xiangshenke formula can inhibit the SGC-7901 cell epithelial-mesenchymal transition by regulating the GSK3β/β-Catenin signaling pathway， and suppress their migrtaion and invasion ability. It plays an important role in adjuvant therapy after gastric cancer surgery. |
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