| 王旭，邵鑫，许海燕，叶丽芳，徐奚如，杨晓青.糖脂清对糖尿病大鼠海马内质网应激相关因子GRP78、CHOP、Caspase-12表达的影响[J].南京中医药大学学报(社会科学版),2019,35(1):73-77. |
| 糖脂清对糖尿病大鼠海马内质网应激相关因子GRP78、CHOP、Caspase-12表达的影响 |
| Effects Tangzhiqing on the Expression of Endoplasmic Reticulum Stress Related Factors GRP78， CHOP and Caspase-12 in the Hippocampus of Diabetic Rats |
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| DOI： |
| 中文关键词: 关键词：糖脂清 2型糖尿病 内质网应激 GRP78 CHOP Caspase-12 |
| 英文关键词: Tangzhiqing type 2 diabete endoplasmic reticulum stress GRP78 CHOP Caspase-12 |
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| 中文摘要: |
| 目的 通过观察糖脂清对糖尿病大鼠海马组织的内质网应激相关因子GPR78、CHOP、Caspase-12的影响，探讨其在改善糖尿病大鼠认知功能的作用机制。方法 采用高糖高脂饲料联合小剂量链脲佐菌素腹腔注射复制2型糖尿病大鼠模型，并给予糖脂清高、中、低剂量组干预8周后，采用ELISA法检测血清空腹葡萄糖（FPG）、空腹胰岛素（FINS），计算稳态模型的胰岛素抵抗指数（HOMA-IR）、胰岛素敏感指数（ISI）；qPCR和Western blot法检测海马组织内质网应激相关因子GPR78、CHOP、Caspase-12的mRNA及蛋白表达水平。结果 糖脂清各剂量组均能够显著降低FPG及HOMA-IR（ P <0.01），除低剂量组外，糖脂清均可显著提高ISI（ P <0.01），3组FINS水平无显著性差异（ P >0.05）。与模型组比较，糖脂清低、中、高3个剂量组GRP78 mRNA和蛋白相对表达均显著增加，CHOP、Caspase-12 mRNA表达均减少（ P <0.01），中、高剂量组CHOP、Caspase-12蛋白表达显著减少（ P <0.01）。结论 糖脂清能够控制血糖，改善胰岛素抵抗，增加胰岛素敏感性，同时能够调节海马内质网应激相关因子GRP78、CHOP、Caspase-12 mRNA和蛋白的表达。 |
| 英文摘要: |
| OBJECTIVE To observe the effect of Tangzhiqing on the endoplasmic reticulum stress related factors GPR78， CHOP and Caspase-12 in hippocampus of diabetic rats， and to explore its mechanism of improving cognitive function in diabetic rats. METHODS The model of type 2 diabetes mellitus rats was established by intraperitoneal injection of low-dose of streptozotocin combined with high-sugar and high-fat diet， After 8 weeks of intervention with high， medium and low-dose of Tangzhiqing， serum fasting glucose (FPG) and fasting insulin (FINS) were detected by ELISA， the index of insulin resistance (HOMA-IR) and insulin sensitivity index (ISI) were calculated; the expression levels of endoplasmic reticulum stress related genes GPR78， CHOP and Caspase-12 mRNA and protein in hippocampus were detected by qPCR and Western blot assay. RESULTS Different dosages of Tangzhiqing significantly decreased FPG and HOMA-IR ( P <0.01); except the low dose group， Tangzhiqing significantly improved ISI ( P <0.01); no significant difference existed in FPG level among the 3 groups ( P >0.05). Compared to the model group， the relative expression of GRP78 mRNA and protein in the 3 groups of Tangzhiqing increased significantly， and the expression of CHOP and Caspase-12 mRNA in these 3 groups decreased ( P <0.01). CHOP and Caspase-12 in middle and high dose group decreased significantly ( P <0.01). CONCLUSION Tangzhiqing can control blood sugar， improve insulin resistance， increase insulin sensitivity and regulate the expression of GRP78， CHOP， Caspase-12 in hippocampus. |
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