文章摘要
刘敏，刘春亮，梁国强，姚霏，张晓迪，陈婷，袁雅琴，吴士良.追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制[J].南京中医药大学学报(社会科学版),2019,35(1):63-67.
追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制
Reverse Effect and Mechanism of Effective Components of Zhuidu Fang on Triple Negative Breast Cancer MDA-MB-231 Drug-Resistant Cells
  
DOI：
中文关键词: 关键词：追毒方  三阴性乳腺癌MDA-MB-231耐药细胞  β3GnT8  逆转耐药
英文关键词: Zhuidu Fang  triple negative breast cancer MDA-MB-231 drug-resistance cells  β3GnT8  reverse the drug-resistance
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作者单位
刘敏1，刘春亮2，梁国强1，姚霏1，张晓迪1，陈婷1，袁雅琴2，吴士良2 1.南京中医药大学附属苏州市中医医院，江苏 苏州 215009；2.苏州大学生物化学和分子生物基础医学院，江苏 苏州 215123 
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中文摘要:
      目的 基于糖基转移酶β3GnT8探讨追毒方逆转三阴性乳腺癌MDA-MB-231细胞耐药的作用机制。方法 通过MTT法观察追毒方对MDA-MB-231耐药细胞增殖的抑制作用，通过转染β3GnT8得到高表达β3GnT8的MDA-MB-231/TAX细胞株，采用Western blot观察糖基转移酶β3GnT8和耐药蛋白BCRP、P-gp的表达，通过凝集素流式细胞术观察转染β3GnT8 MDA-MB-231/TAX细胞膜表面β3GnT8的荧光强度。结果 追毒方有效成分能明显抑制三阴性乳腺癌MDA-MB-231耐药细胞的增殖，作用72 h抑制率达到50.16%，能有效下调β3GnT8和耐药蛋白BCRP、P-gp的表达，并且β3GnT8的表达与BCRP、P-gp呈正相关。结论 追毒方可通过下调糖基转移酶β3GnT8，从而降低耐药蛋白BCRP、P-gp的表达，逆转三阴性乳腺癌MDA-MB-231细胞耐药性。
英文摘要:
      OBJECTIVE Based on glycosyltransferase β3GnT8， to investigate the mechanism of reverse effect on triple negative breast cancer MDA-MB-231 drug-resistance cells by Zhuidu Fang. METHODS The inhibition of MDA-MB-31 drug-resistance cells proliferation by Zhuidu Fang was detected by MTT assay. By interference and transfection of β3GnT8， high and low expressed β3GnT8 MDA-MB-231/TAX cell lines were obtained. Western blot was used to detect the protein expression of β3GnT8， and drug resistance protein BCRP and P-gp. Lectin flow cytometry was used to detect β3GnT8 fluorescence intensity on β3GnT8 transfected MDA-MB-231/TAX cells. RESULTS Zhuidu Fang effectively inhibited the proliferation of MDA-MB-231/TAX cells， and the inhibitory rate was 50.16% after 72 h. Zhuidu Fang significantly downregulated the protein expression of β3GnT8， BCRP and P-gp. The protein expression of β3GnT8 showed a positive correlation with BCRP and P-gp. CONCLUSION Zhuidu Fang can reverse the drug-resistance of MDA-MB-231 cell lines， which is associated with the downregulation of the expression of β3GnT8， BCRP and P-gp.
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