文章摘要
李维薇，汪受传，单进军，谢彤，林丽丽，贺丽丽，杜丽娜，杨燕.基于UHPLC-MS的代谢组学技术研究婴儿巨细胞病毒肝炎湿热内蕴证[J].南京中医药大学学报(社会科学版),2019,35(1):39-46.
基于UHPLC-MS的代谢组学技术研究婴儿巨细胞病毒肝炎湿热内蕴证
Using Metabolomics to Explore the Essence of Damp Heat Accumulation Syndrome of Infantile Human Cytomegalovirus Hepatitis Based on UHPLC-MS System
  
DOI：
中文关键词: 关键词：代谢组学  湿热内蕴证  生物标志物  超高效液相色谱-质谱联用  婴儿巨细胞病毒肝炎
英文关键词: metabolomics  damp heat accumulation syndrome  biomarker  UHPLC-MS  infantile cytomegalovirus hepatitis
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作者单位
李维薇1，2，汪受传1，单进军2，谢彤2，林丽丽2，贺丽丽3，杜丽娜4，杨燕4 1.南京中医药大学附属医院，江苏 南京 210029；2.南京中医药大学中医儿科学研究所，江苏 南京 210023；3.南京中医药大学附属中西医结合医院，江苏 南京 210028
4.首都医科大学附属北京儿童医院，北京 100045 
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中文摘要:
      目的 利用代谢组学技术探讨人巨细胞病毒（HCMV）肝炎湿热内蕴证的证候实质。方法 收集20例HCMV肝炎湿热内蕴证的患儿和20例正常对照组婴儿。采用超高效液相-线性离子阱/静电场轨道阱质谱联用技术对这些受试者的血浆及尿液样本进行代谢组学研究。通过正交偏最小二乘法对这些代谢数据进行分析，同时采用受试者工作曲线评价潜在生物标志物的特异性和敏感性。结果 共检测到1 076个血浆代谢物和414个尿液代谢物。最终共鉴定出22个血浆代谢物和7个尿液代谢物，涉及鞘脂、甘油磷脂、组氨酸、甘油酯和脂肪酸代谢，其中鞘磷脂和甘油三酯可作为HCMV肝炎湿热内蕴证潜在的生物标记物。结论 代谢物和生物标志物的确认为探讨HCMV肝炎湿热内蕴证的证候实质提供依据。
英文摘要:
      OBJECTIVE Using metabolomics to explore the essence of damp heat accumulation syndrome of infantile human cytomegalovirus hepatitis. METHODS 20 infantile HCMV hepatitis patients with damp heat accumulation syndrome and 20 healthy infants were recruited for investigation. Plasma and urine samples were analyzed using ultra-high-performance liquid chromatography-linear trap quadruple/orbitrap mass spectrometry (UHPLC-LTQ/Orbitrap-MS) to determine the alterations in metabolomic profiles of this syndrome. Orthogonal partial least squares-discriminate analysis (OPLS-DA) was applied to analyze the UHPLC-MS data obtained from these samples. The specificity and sensitivity of potential biomarkers were assessed according to the area under the receiver operator characteristic (ROC) curves. RESULTS The metabolomic analysis yielded 1076 plasma compounds and 414 urine compounds. Among them， 22 plasma and 7 urine differential metabolites relating to sphingolipid， glycerophospholipid， histidine， glycerolipid， and fatty acid metabolism were identified. Sphingomyelin and triglycerides were screened as potential biomarkers and showed excellent discriminant performance. CONCLUSION This research can provide insights into the essence of HCMV hepatitis damp heat accumulation syndrome.
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