文章摘要
张艺，王江荟，.越鞠甘麦大枣汤调节小鼠海马PKA-CREB-BDNF信号通路的抗抑郁作用[J].南京中医药大学学报(社会科学版),2018,34(6):572-577.
越鞠甘麦大枣汤调节小鼠海马PKA-CREB-BDNF信号通路的抗抑郁作用
Antidepressant-Like Effects of Yueju Ganmai Dazao Decoction Associated with PKA-CREB-BDNF Signaling Pathway in the Hippocampus of Mice
  
DOI：
中文关键词: 关键词：越鞠甘麦大枣汤  抗抑郁样作用  PKA  CREB  BDNF
英文关键词: Yueju Ganmai Dazao Decoction  antidepressant-like effects  PKA  CREB  BDNF
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作者单位
张艺1，王江荟1， 1.南京中医药大学基础生物医学科学院转化系统生物学与神经科学中心，中医脑病重点实验室，江苏 南京 210023；2.南京中医药大学心理学院，江苏 南京 210023 
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中文摘要:
      目的 探讨越鞠甘麦大枣汤（YG）的抗抑郁功效及其与PKA-CREB-BDNF信号通路的关联。方法 强迫游泳（FST）检测越鞠甘麦大枣汤单次给药后抗抑郁的时效性。慢性不可预测模型（CMS）进一步检测越鞠甘麦大枣汤在抑郁模型上的抗抑郁作用。蛋白印迹表达法检测越鞠甘麦大枣汤单次给药30 min和24 h后海马中PKA、CREB、BDNF的蛋白表达。结果 YG在FST中显示出持久的抗抑郁潜力，并且快速逆转了慢性应激小鼠的抑郁样行为。在慢性小鼠模型中，海马中PKA，CREB和BDNF的表达显著上调。结论 越鞠甘麦大枣汤（YG）显示出快速和持久的抗抑郁样作用，其抗抑郁功能与PKA-CREB-BNDF通路有关。
英文摘要:
      OBJECTIVE To explore the antidepressant effect of Yueju Ganmai Dazao Decoction (YG) and its association with PKA-CREB-BDNF signaling pathway. METHODS Forced swimming (FST) was used to detect the time-effect of anti-depression after a single administration of YG.The chronic unpredictable model (CMS) further tested the antidepressant effect of YG on the depression model.Western blot expression was used to detect the expression of PKA， CREB and BDNF in hippocampus at 30 min and 24 h after single administration of YG. RESULTS YG showed long-lasting antidepressant potential in FST， meanwhile it also significantly and rapidly reversed behavioral deficits in chronically stressed mice. In the chronic mouse model， the expression of PKA， CREB and BDNF in the hippocampus was significantly up-regulated. CONCLUSION YG displays rapid and lasting antidepressant-like effect and its function is related to the PKA-CREB-BNDF pathway.
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