GC-MS结合AMDIS和保留指数法分析白术芍药散及其组方饮片中挥发油的组成

徐洋洋1; 2; 蔡皓1; 2*; 段煜1; 2; 裴科3; 范恺磊1; 2; 谢莉1; 2; 曹岗4*

文章摘要

徐洋洋1，2，蔡皓1，2*，段煜1，2，裴科3，范恺磊1，2，谢莉1，2，曹岗4*.GC-MS结合AMDIS和保留指数法分析白术芍药散及其组方饮片中挥发油的组成[J].南京中医药大学学报(社会科学版),2018,34(4):409-415.

GC-MS结合AMDIS和保留指数法分析白术芍药散及其组方饮片中挥发油的组成

Comparative Investigation on Composition of Essential Oils of Baizhu Shaoyao San and Medicinal Herbs Contained in Baizhu Shaoyao San by GC-MS Combined with AMDIS and Retention Index

DOI：

中文关键词: 白术芍药散挥发油配伍 GC-MS AMDIS 保留指数

英文关键词: Baizhu Shaoyao San essential oils compatibility GC-MS AMDIS retention index

基金项目:

作者	单位
徐洋洋1，2，蔡皓1，2，段煜1，2，裴科3，范恺磊1，2，谢莉1，2，曹岗4	1.南京中医药大学药学院，江苏南京 210023 2.南京中医药大学，国家教育部中药炮制规范化及标准化工程研究中心，江苏南京 210023 3.山西中医药大学制药与食品工程学院，山西太原 030024 4.浙江中医药大学中药炮制技术研究中心，浙江杭州 310053

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中文摘要:

目的研究白术芍药散各组方饮片组成复方前后挥发油的组成成分变化。方法通过水蒸气蒸馏法提取白术芍药散及其组方饮片（炒白术、炒白芍、炒陈皮、防风）中的挥发油，经GC-MS测定得到总离子流图谱，利用自动质谱退卷积定性系统（AMDIS）和保留指数（RI）对检测结果进行定性分析，应用峰面积归一法计算各化合物的相对含量。结果研究结果表明白术芍药散的挥发油主要来源于炒白术、炒陈皮和防风，组方饮片经配伍后，其挥发油的成分与比例发生明显变化。结论该研究可为白术芍药散组方的物质基础和配伍机理的挖掘奠定基础。

英文摘要:

OBJECTIVE The experiment was designed to investigate the changes of the chemical constituents from the essential oils of Baizhu Shaoyao San and the medicinal herbs contained in Baizhu Shaoyao San. METHODS The essential oils from Baizhu Shaoyao San and the medicinal herbs (stir-baked Atractylodis Macrocephalae Rhizoma， stir-baked Paeoniae Radix Alba， stir-baked Citri Reticulatae Pericarpium， Saposhnikoviae Radix) contained in Baizhu Shaoyao San were extracted by steam distillation method. GC-MS combined with automated mass spectral deconrolution and identification system (AMDIS) and retention index were employed to identify the chemical components of the essential oils and the relative amounts of these chemical constituents were calculated by peak area normalization method. RESULTS The results showed that the essential oils of Baizhu Shaoyao San were mainly derived from stir-baked Atractylodis Macrocephalae Rhizoma， stir-baked Citri Reticulatae Pericarpium， and Saposhnikoviae Radix. The quantity and quality of the volatile constituents from the medicinal herbs contained in Baizhu Shaoyao San experienced apparent changes after their compatibility. CONCLUSION Our investigation can make a good foundation for the material basis and the compatibility mechanism of Baizhu Shaoyao San.

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