文章摘要
李恋曲1，季律1，2，魏盼1，2，袁为远1，2，包凯帆1，华永庆1，吴鹏1，洪敏1*，江国荣2*.麻黄-甘草药对抑制Th2型变应性接触性皮炎的作用及机制探讨[J].南京中医药大学学报(社会科学版),2018,34(3):282-286.
麻黄-甘草药对抑制Th2型变应性接触性皮炎的作用及机制探讨
Effect of Ephedra-Licorice on Th2 Allergic Contact Dermatitis Through Inhibiting Expression of TSLP
  
DOI：
中文关键词: 麻黄  甘草  TSLP  变应性接触性皮炎  Th2细胞因子
英文关键词: ephedra  licorice  TSLP  allergic contact dermatitis  Th2 cytokines
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作者单位
李恋曲1，季律1，2，魏盼1，2，袁为远1，2，包凯帆1，华永庆1，吴鹏1，洪敏1*，江国荣2* 1.南京中医药大学药学院，科技部国家规范化中药药理实验室，江苏省中药药效与安全性评价重点实验室，江苏 南京 210023；2.苏州市中医医院药剂科，江苏 苏州 215009 
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中文摘要:
      研究麻黄-甘草药对对Th2型变应性接触性皮炎（Allergic contact dermatitis，ACD）的影响及机制。方法 采用不同浓度异硫氰酸荧光素（FITC）诱导BALB/c小鼠建立Th2型ACD模型及诱导初期ACD模型。HE染色观察小鼠耳组织病理学变化，测量小鼠耳肿胀，ELISA法检测小鼠耳组织Th2细胞因子水平及胸腺基质淋巴细胞生成素（Thymic stromal lymphopoietin，TSLP）蛋白水平，用qPCR检测小鼠耳组织TSLP mRNA水平，Poly(I∶C)和TNF-α联合刺激HaCaT细胞，检测细胞分泌的TSLP蛋白水平。结果 Th2型ACD模型中，与模型组小鼠比较，麻黄-甘草2.34g/kg和7.02g/kg组均明显抑制小鼠耳肿胀，减少耳组织炎性细胞浸润；麻黄-甘草2个剂量组均显著降低耳匀浆中IL-4水平，麻黄-甘草7.02g/kg组明显降低IL-13水平。诱导初期Th2型ACD模型中，与模型组比较，麻黄-甘草药对显著降低耳匀浆TSLP的基因及蛋白表达，体外麻黄-甘草药对及其主要单体成分同样抑制TSLP的分泌。结论 麻黄-甘草药对具有抑制过敏性炎症的作用，其作用机制可能是通过减少TSLP的分泌。
英文摘要:
      OBJECTIVE To investigate the effect and mechanism of ephedra-licorice drug pair on Th2 allergic contact dermatitis (ACD) model mice. METHODS Th2 ACD model and early phase of ACD model were established on BALB/c mice by using different concentrations of fluorescein isothiocyanate(FITC). HE staining was used to observe the histopathological changes of ears in mice. Ear swelling of mice were measured. The levels of Th2 cytokines and thymic stromal lymphocytes were measured by enzyme-linked immunosorbent assay (ELISA) were detected by real-time quantitative PCR (qPCR). The expressions of TSLP mRNA were detected by Poly (I∶ C) and TNF-α stimulated HaCaT cells. RESULTS In Th2 type ACD model， compared with the model group， ephedra-licorice 2.34g/kg and 7.02g/kggroups significantly inhibited ear swelling in mice and decreased inflammatory cell infiltration in the ear tissue. Both doses of ephedra-licorice group significantly reduced the level of IL-4 in the ear homogenate， and ephedra-licorice 7.02g/kg group significantly reduced IL-13 levels. Compared with the model group， ephedra-licorice significantly reduced the mRNA and protein levels of TSLP in the early stage of Th2 ACD model. In vitro， ephedra-licorice and the main monomers of ephedra and licorice also inhibited the secretion of TSLP. CONCLUSION Ephedra-licorice can inhibit allergic inflammation and its mechanism may be through the reduction of TSLP secretion.
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