基于UFLC-Q-TOF/MS分析黄芪-丹参药对化学成分研究

祁晓霞; 董宇; 单晨啸; 项洋洋; 王新东

文章摘要

祁晓霞，董宇，单晨啸，项洋洋，王新东.基于UFLC-Q-TOF/MS分析黄芪-丹参药对化学成分研究[J].南京中医药大学学报(社会科学版),2017,33(1):93-96.

基于UFLC-Q-TOF/MS分析黄芪-丹参药对化学成分研究

Analysis of the Main Components in Herbal Pair: Astragali Radix and Salviae Miltiorrhizae by UFLC-Q-TOF/MS

DOI：

中文关键词: 黄芪-丹参 UFLC-Q-TOF/Ms 化学成分

英文关键词: Astragali Radix and Salviae Miltiorrhizae UFLC-Q-TOF/MS chemical compounds

基金项目:

作者	单位
祁晓霞，董宇，单晨啸，项洋洋，王新东	1.南京中医药大学科技与产业处，江苏南京 210023 2.南京中医药大学药学院，江苏南京 210023；3.南京中医药大学附属中西医结合医院，江苏南京 210028

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中文摘要:

目的应用UFLC-Q-TOF/MS对黄芪-丹参药对中的化学成分进行鉴定。方法采用Thermo BDS C₁₈(3μm，150mm×2.1mm)色谱柱，以0.1%甲酸水-0.1%乙腈为流动相进行梯度洗脱，流速为0.3mL/min，柱温40℃，进样量5μL。离子源为电喷雾离子源（ESI），负离子模式下采用飞行时间质谱采集数据，全扫描质量范围为m/z 100~1500。依据保留时间、精确分子量、二级质谱裂解碎片，结合对照品比对初步鉴定化合物。结果从黄芪-丹参药对中初步鉴定或推断出28个化合物，其中丹参中15个化合物，黄芪中11个化合物，另有2个未知化合物。包括皂苷类、有机酸类、黄酮类和醌类化合物。结论该UFLC-Q-TOF/MS可全面、快速分析黄芪-丹参药对中的化学成分，研究结果为对其进行活性成分研究和质量控制提供了一定参考。

英文摘要:

OBJECTIVE To identify the main chemical components in herbal pair:Astragali Radix and Salviae Miltiorrhizae by UFLC-Q-TOF/MS. METHODS Thermo BDS C₁₈ (3μm，150mm×2.1mm) column was employed for the separation with acetonetrile-water (0.1% formic acid) as mobile phase using a gradient elution program. The flow rate was set as 0.3mL/min， the temperature of column was 40℃ with injection volume of 5μL. Electrospray ionization (ESI)-Q-TOF/MS in negative ion mode was performed to obtain the MS spectra with full mass scan m/z ranged from 100 to 1500. Chemicals were identified based on the retention time， high-resolution mass information， MS/MS fragmentation behaviors and standards. RESULTS 28 components were identified or tentatively characterized in herbal pair:Astragali Radix and Salviae Miltiorrhizae， including 15 components in Salviae Miltiorrhizae， 11 compounds in Astragali Radix， and 2 unknown compounds. And these chemicals could be classified as triterpenes， organic acids， flavonoids and quinines. CONCLUSION This method can rapidly and accurately identify the main compounds in herbal pair:Astragali Radix and Salviae Miltiorrhizae， which can help us for the quality control and further phytochemical studies of Astragali Radix and Salviae Miltiorrhizae. in traditional Chinese medicine.

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