| 徐飞，于慧，陆彩，吴启南，谷巍，陈军.泽泻醇类化合物调血脂作用及分子机制的研究[J].南京中医药大学学报(社会科学版),2016,32(5):451-455. |
| 泽泻醇类化合物调血脂作用及分子机制的研究 |
| Study on Alisols Hypolipidemic Effect and Molecular Mechanism |
| 投稿时间：2016-03-15 修订日期：2016-06-22 |
| DOI： |
| 中文关键词: 泽泻醇类化合物 TC、TG、HDL-C LCAT 同源建模 分子模拟 |
| 英文关键词: Alisol acetates TC,TG,HDL-C LCAT homology modeling molecular modeling |
| 基金项目: |
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| 摘要点击次数: 1081 |
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| 中文摘要: |
| 目的 研究泽泻调脂效应物质泽泻醇类化合物23-乙酰泽泻醇B、24-乙酰泽泻醇A对高脂小鼠调血脂作用及其分子机制。方法 建立高脂小鼠模型，检测泽泻醇给药后的总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C），检测了脂代谢关键酶卵磷脂胆固醇酰基转移酶（LCAT）的活性，通过同源建模得到LCAT分子结构，采用分子模拟进行了泽泻醇与LCAT相互作用的研究。结果 泽泻醇降低TG、TC水平，升高HDL-C水平，起到调血脂作用。泽泻醇降低LCAT活性，与LCAT的结合区域在Leu201~Phe305范围内。结论 泽泻醇升高HDL-C的机制可能非通过提高LCAT活性实现，Leu201~Phe305区域可能为其抑活位置。Ile227、Arg217、Gly229 3个氨基酸可能是2者与该蛋白相互作用的关键氨基酸残基。 |
| 英文摘要: |
| OBJECTIVE To study the different effects and molecular mechanism of Alisol acetates(alisol B 23-acetate and alisol A 24-acetate)on the decrease of the hyperlipidemia of fat mice. METHODS Established a hyperlipidemic mice model，measured the level of TC， TG and HDL-C after the administration of Alisol acetates，detected the activity of LCAT， which is a key enzyme in lipid metabolism，obtained the molecular structure of LCAT by homology modeling and studied the interaction between LCAT and Alisol acetates using molecular modeling. RESULTS Alisol acetates played a hypolipidemic effect by reducing TG， TC level and increasing HDL-C level. lisol acetates decreased the LCAT activity. Alisol acetates binding regions are in the range of Leu201~Phe305. CONCLUSION Alisol acetates elevated HDL-C mechanism may not be by modulating LCAT activity. The region of Leu201~Phe305 may be the suppression positions. Ile227， Arg217， Gly229 may be the critical amino acid residues for the interaction. |
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