| 张农山1，戴国梁1，冯丽雯2，李长印1，黄国栋2，刘史佳1*，居文政1*.LC-MS/MS研究柠檬苦素在大鼠肠道内的分布[J].南京中医药大学学报(社会科学版),2016,32(4):379-382. |
| LC-MS/MS研究柠檬苦素在大鼠肠道内的分布 |
| Study on Limonin Distribution in Rat Intestine by LC-MS/MS Method |
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| DOI： |
| 中文关键词: 柠檬苦素 肠道分布 LC-MS/MS 组织浓度 药代动力学 |
| 英文关键词: limonin intestine distribution LC-MS/MS tissue concentration pharmacokinetics |
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| 中文摘要: |
| 目的 考察柠檬苦素在大鼠肠道不同部位的吸收情况。方法 大鼠灌胃给予柠檬苦素80mg/kg，分别于给药后2、3、4、6、8h分离并保存大鼠十二指肠、小肠、直肠组织。采用LC-MS/MS液质联用仪测定大鼠肠道中柠檬苦素的浓度。结果 柠檬苦素在大鼠肠道各部分组织中均有分布，大部分肠道组织在给药后3~4h可达到峰浓度，柠檬苦素在大鼠的小肠中药物浓度最高，在十二指肠和直肠中浓度次之。结论 说明柠檬苦素在大鼠肠道整体分布较广，局部浓度较高，可能通过局部肠道吸收而直接在大鼠肠道靶组织发挥疗效。 |
| 英文摘要: |
| OBJECTIVE Integral animal experiment was employed to investigate the limonin distribution in different parts of rats’ intestine. METHODS The concentration of limonin in different parts of the intestinal was detected by LC-MS/MS method. After the rats were given the limonin 80mg/kg ig， the drug concentrations in the duodenum， small intestine and rectum were detected at 2，3，4，6 and 8h. RESULTS Limonin was highly distributed in different parts of the intestinal tissue. Most of intestinal tissue reached peak concentration at 3~4h after administration. The drug concentration in the duodenum and rectum was the highest， with duodenum being the second. CONCLUSION Limonin has a wide distribution and high concentration in rat intestine， and it plays a direct role in the treatment of hemorrhoids in intestine. |
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