| 郭秀霞1，唐德才1*，臧文华2，刘敏1，戴建国1，张硕1，尹刚1*.人卵巢癌HO-8910原位移植瘤动物模型建立及分期研究[J].南京中医药大学学报(社会科学版),2016,32(4):356-358. |
| 人卵巢癌HO-8910原位移植瘤动物模型建立及分期研究 |
| Establishment and Staging Studies of Human Ovarian Cancer HO-8910 Orthotopic Xenograft Animal Models |
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| DOI： |
| 中文关键词: 卵巢癌 HO-8910细胞株 原位移植瘤 分期 |
| 英文关键词: ovarian cancer HO-8910 cell line orthotopic xenograft stage |
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| 中文摘要: |
| 目的 建立HO-8910卵巢癌荧光原位移植瘤模型，并进行分期研究。方法 将人卵巢癌HO-8910细胞株在细胞培养瓶中培养至10代，选荧光稳定表达的细胞注射到裸鼠皮下，待成瘤后通过显微外科手术将体积为1mm3肿瘤块植入到实验裸鼠卵巢黏膜上，在活体成像技术下观察肿瘤的生长和转移情况，并应用病理学检测方法对肿瘤组织及附近组织进行观察。结果 活体成像技术成功追踪了肿瘤的发展与转移，病理结果显示肿瘤细胞的转移趋势，2者与中医临床卵巢癌分期相结合，确立了卵巢癌分期。结论 成功建立卵巢癌原位移植瘤模型，再现了肿瘤临床演变过程。其3期分期为肿瘤研究及抗肿瘤药物疗效判定研究提供了实验基础。 |
| 英文摘要: |
| OBJECTIVE To establish an ovarian cancer xenograft model by fluorescence and study of the cancer staging. METHODS The human ovarian cancer HO-8910 cells were cultured for 10 generations in the cell culture dish， and the cells that could stably express fluorescence were selected and injected into nude mice. When the tumor grew to a mass volume of 1mm3， it was implanted into the ovarian mucous membrane of nude mice through micro-surgery. The tumor growth and metastasis were observed by in vivo imaging technique， and the tumor tissue and surrounding tissues were observed through pathology detection methods. RESULTS In vivo imaging technology successfully traced the development and metastasis of tumor. Pathological examination showed tumor cell metastasis trend. Both of them combining with TCM clinical ovarian cancer staging determined the ovarian cancer staging. CONCLUSION The experiment successfully establishes ovarian cancer orthotopic transplantation model and represents the evolution of clinical oncology.The stages will provide some experimental basis for the study of tumor and the evaluation criterion of antitumor drug effect. |
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