| 尹刚1，唐德才1*，戴建国1，臧文华1，吴晓宇2.黄芪、莪术配伍对人卵巢癌HO-8910原位移植瘤组织中MMP2和VEGF表达的影响[J].南京中医药大学学报(社会科学版),2014,30(1):53-56. |
| 黄芪、莪术配伍对人卵巢癌HO-8910原位移植瘤组织中MMP2和VEGF表达的影响 |
| Effect of Compatibility of Astragalus Plus Zedoary on the Expression of MMP2 and VEGF in Orthotopic Transplantation Tumor Tissue of HO-8910 Ovarian Carcinoma in Situ |
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| DOI： |
| 中文关键词: 黄芪 莪术 卵巢癌 MMP2 VEGF |
| 英文关键词: Astragali Zedoary ovarian cancer MMP2 VEGF |
| 基金项目: |
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| 中文摘要: |
| 目的 观察黄芪、莪术配伍对人卵巢癌HO-8910原位移植瘤抑瘤效果以及肿瘤组织中MMP2和VEGF表达的影响。方法 选取已建立荧光蛋白转染的HO-8910卵巢癌原位癌动物模型（5组），分为模型组，阳性对照组（顺铂），黄芪组，莪术组，黄芪、莪术配伍组（按照2:1），动态测量各组肿瘤体积增长情况，观察抑瘤效果。用免疫组织化学法检测移植瘤组织中MMP2和VEGF蛋白的表达并计算表达率。结果 黄芪、莪术配伍组以及顺铂组的移植瘤体积和瘤质量均小于模型组（P<0.05），黄芪、莪术配伍组移植瘤体积和瘤质量小于顺铂组、莪术组以及黄芪组（P<0.05），莪术组移植瘤体积和瘤质量与模型组相比较无统计学差异（P>0.05）。黄芪、莪术配伍组移植瘤体积和瘤质量小于顺铂组（P<0.05）；与模型组比较黄芪组，莪术组，黄芪、莪术配伍组和顺铂组移植瘤组织中MMP2和VEGF蛋白表达水平平均减弱（P<0.05）。结论 黄芪、莪术配伍使用在实验周期内对人卵巢癌HO-8910抑瘤效果明显（P<0.05），其作用机制可能与下调MMP2和VEGF蛋白的表达有关。 |
| 英文摘要: |
| OBJECTIVE To observe the effect of Radix Astragali， Rhizoma Zedoariae and antitumor effect and the effect of MMP2 and VEGF expression in tumor tissues of human ovarian cancer HO-8910 orthotopic transplantation tumor. METHODS elect the HO-8910 ovarian carcinoma in situ animal models have been established fluorescent protein transfection(5 grope)，Including model group， positive control group (Shun Bo)， Astragalus group， Zedoary group， Astragalus，zedoary turmeric compatibility group (on the basis of 2∶1). Dynamic measurement of each tumor volume， tumor metastasis， tumor inhibitory effect. The protein expression of MMP2 and VEGF were deteced by immunohistochemical method. RESULTS Astragalus root， zedoary turmeric compatibility group and cisplatin group， tumor volume and tumor weight were lower than model group(P<0.05)， Astragalus， zedoary turmeric compatibility group tumor volume and tumor weight less than cisplatin group，Curcuma group and Astragalus group， Zedoary group of tumor volume and tumor weight compared with the model group（P>0.05）， Astragalus root， zedoary turmeric compatibility group tumor volume and tumor weight less than cisplatin group(P<0.05)， Compared with the model group the level of MMP2 and VEGF protein expression of average weakened Astragalus， zedoary turmeric compatibility group Astragalus group， Zedoary group and cisplatin group in transplanted tumor tissue(P<0.05). CONCLUSION In the experimental period，Astragalus root， zedoary turmeric compatibility which has obvious anti-tumor effect on human ovarian cancer HO-8910(P<0.05)，The action mechanism of the expression may be associated with the downregulation of MMP 2 and VEGF protein. |
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