| 冯颖，刘晓丹，潘涛.大株红景天对缺氧/复氧大鼠原代心肌细胞线粒体功能及线粒体动力学的影响[J].南京中医药大学学报(社会科学版),2018,34(2):168-172. |
| 大株红景天对缺氧/复氧大鼠原代心肌细胞线粒体功能及线粒体动力学的影响 |
| Effects of Sofren on Mitochondrial Function and Dynamics of Hypoxia/Reoxygenation Cardiomyocytes |
| 投稿时间：2017-09-22 |
| DOI： |
| 中文关键词: 大株红景天 缺氧/复氧 心肌细胞 三磷酸腺苷 活性氧簇 线粒体膜电位 OPA1；Drp1；p-DRP1 |
| 英文关键词: sofren hypoxia/reoxygenation cardiomyocytes ATP ROS MMP OPA1 Drp1 p-DRP1 |
| 基金项目: |
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| 中文摘要: |
| 通过观察大株红景天对缺氧/复氧（Hypoxia/reoxygenation，H/R）心肌细胞线粒体功能及线粒体动力学的影响，探讨其抗心肌细胞H/R损伤的可能机制。方法 分离并培养新生SD大鼠原代心肌细胞，选符合实验要求的心肌细胞，随机分为5组：空白对照组、缺氧/复氧组（H/R组）、红景天前干预组（S+H/R组）、红景天后干预组（H/R+S组）、红景天前后干预组（S+H/R+S组）。实验结束后多功能酶标仪测定心肌细胞内三磷酸腺苷（ATP）水平、活性氧簇（ROS）含量，JC-1法测定心肌细胞线粒体膜电位（MMP），Western blot法检测线粒体动力学相关蛋白视神经萎缩蛋白（OPA1）、动力相关蛋白1（DRP1）及磷酸化动力相关蛋白1（p-DRP1）的表达水平。结果 S+H/R组、H/R+S组及S+H/R+S组H/R心肌细胞内ATP含量均增加，ROS水平降低，并抑制MMP的降低，上调OPA1蛋白表达，抑制p-Drp1蛋白表达（P＜0.05）；S+H/R+S组分别与S+H/R组、H/R+S组组间比较，ATP、ROS、MMP水平变化及p-Drp1表达量均具有统计学差异（P＜0.05），而OPA1蛋白的表达无明显差异；S+H/R组、H/R+S组及S+H/R+S组组间比较，Drp1的表达量无明显差异。结论 大株红景天对H/R损伤的心肌细胞有保护作用，其机制可能与改善受损心肌细胞线粒体功能障碍、稳定线粒体动力学平衡有关，值得进一步探讨。 |
| 英文摘要: |
| OBJECTIVE To explore the effects of Sofren on mitochondrial dysfunction and dynamic unbalance induced by H/R injury. METHODS Primary neonatal rat cardiomyocytes were cultured and randomly assigned to five groups based on the sofren that was used and the treatment simulating reperfusion injury: control group， H/R group， sofren preconditioning+H/R group (S+H/R)， H/R+sofren postconditioning group (H/R+S)， sofren pre- and postconditioning +H/R group (S+H/R+S). The intracellular adenosine triphosphate (ATP) level， reactive oxygen species (ROS) accumulation was measured by multifunctional microplate reader，mitochondrial membrane potential (MMP) was measured though JC-1， mitochondrial optic atrophy(OPA1)， dynamin-related protein 1(Drp1) and phosphorylation dynamin-related 1 (p-Drp1) was measured through Western blot in each group. RESULTS The ROS accumulation was inhibited， MMP and higher ATP level was maintained， higher OPA1 and lower p-DRP1 was expressed，preconditioning or postconditioning or pre- and postconditioning by sofren (P<0.05); there is statistical difference of ATP， ROS and MMP values and p-DRP1 expression between S+H/R+S group and S+H/R group，as well as S+H/R+S group and H/R+S， while no statistical difference of OPA1 expression; there is no statistical difference of DRP1 expression between all the five groups. CONCLUSION These results showed that sofren exerted a protective effect on cardiomyocytes injuried by H/R， and the machanism may correlate with rescuing H/R-induced myocardial mitochondrial dysfunction and dynamic unbalance， and further studies are required. |
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