| 康安，乔广军，张正伟，郭锦瑞，狄留庆，吴勉华，周红光.HPLC法同时测定半枝莲中黄酮和生物碱类成分[J].南京中医药大学学报(社会科学版),2013,29(5):479-481. |
| HPLC法同时测定半枝莲中黄酮和生物碱类成分 |
| Simultaneous Determination of Flavones and Scutebarbatines in Scutellariae barbatae by HPLC |
| 投稿时间：2013-07-03 修订日期：2013-08-21 |
| DOI： |
| 中文关键词: 半枝莲 黄酮 半枝莲生物碱；高效液相色谱法 |
| 英文关键词: Scutellariae barbatae Flavones Scutebarbatines HPLC |
| 基金项目: |
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| 中文摘要: |
| 目的 建立同时测定半枝莲饮片中木犀草素、芹菜素、汉黄芩素、半枝莲生物碱A和半枝莲生物碱B的HPLC法。方法 采用高效液相色谱法，色谱柱为Phenomenon C18柱(250 mm×4.60 mm，5 μm)，以乙腈-水溶液为流动相，梯度洗脱；流速为1.0 mL/min，柱温为30 ℃；木犀草素、芹菜素检测波长为355 nm，汉黄芩素、半枝莲生物碱A和生物碱B检测波长为264 nm。结果 木犀草素、芹菜素、汉黄芩素、半枝莲生物碱A和生物碱B分别在0.0374~2.40 μg、0.0312~2.00 μg、0.0312~2.00 μg、0.0062~0.40 μg和0.0062~0.40 μg内线性关系良好。精密度，重复性及加样回收均符合要求。结论 此方法快速简便，重现性好，可用于半枝莲饮片的质量控制。 |
| 英文摘要: |
| OBJECTIVE To establish HPLC methods for simultaneous determination of luteolin， apigenin， wogonin， scutebarbatine A and scutebarbatine B.METHODS The analytical column was Phenomenex Luna C18(250 mm×4.60 mm， 5 μm). The mobile phase was composed of acetonitrile and water with gradient elution. The flow rate was 1.0 mL/min; The column temperature was 30 ℃. Luteolin and apigenin were detected at 355 nm; wogonin， scutebarbatine A and scutebarbatine B were detected at 264 nm.RESULTS The ranges of calibration curve of luteolin， apigenin， wogonin， scutebarbatine A and scutebarbatine B were 0.037 4~2.40 μg、0.031 2~2.00 μg、0.031 2~2.00 μg、0.006 2~0.40 μg and 0.006 2~0.40 μg， respectively. Also， the intra-batch and inter-batch variations and the average recoveries were all meet the requirement of Chinese Pharmacopoeia.CONCLUSION The method is rapid and simple with a good reproducibility and it can be used for the quality control of herba Scutellariae barbatae. |
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