| 江薇，喻国华，陈建章，唐翔宇，龚晓军，张宏泉，吴娟，王进.隔姜灸对胃癌大鼠VIP和IL-10水平的影响[J].南京中医药大学学报(社会科学版),2013,29(5):466-468. |
| 隔姜灸对胃癌大鼠VIP和IL-10水平的影响 |
| Effects of Ginger Moxibustion on VIP and IL-10 in Gastric Cancer Rats |
| 投稿时间：2013-06-25 修订日期：2013-08-11 |
| DOI： |
| 中文关键词: 胃癌 隔姜灸 VIP IL-10 |
| 英文关键词: gastric cancer ginger moxibustion VIP IL-10 |
| 基金项目: |
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| 摘要点击次数: 754 |
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| 中文摘要: |
| 目的 观察隔姜灸对胃癌大鼠组织血管活性肠肽(VIP)、白介素10(IL-10)的影响。方法 采用(N-甲基-N-硝基-N-亚硝基胍)MNNG建立胃癌模型，造模后随机分为VIP组、VIP受体拮抗剂组、模型组、隔姜灸4组，每组10只，另设空白组10只。VIP组和VIP受体拮抗剂组分别每日皮下注射VIP或VIP受体拮抗剂0.1 μg/mL，空白组给予生理盐水，隔姜灸组隔姜灸中脘、脾俞、胃俞、足三里、内关等穴位，3周后检测胃癌VIP、IL-10含量的变化，通过免疫组织化学和RT-PCR检测胃腺癌组织中VIP蛋白和mRNA表达情况。结果 模型组胃癌组织VIP、IL-10明显增高，隔姜灸组较模型组含量明显降低(P<0.01)，与VIP治疗组比较差异也有显著性(P<0.01)。胃癌组织出现VIP免疫反应阳性细胞，相对正常的胃组织中均未见VIP阳性细胞。胃癌组织中均有VIP mRNA表达。VIP治疗组和隔姜灸均能下调VIP mRNA表达，但隔姜灸效果更佳（P<0.01）。结论 隔姜灸可能通过降低大鼠胃癌组织中VIP、IL-10含量及抑制VIP mRNA表达而达到治疗胃癌的目的。 |
| 英文摘要: |
| OBJECTIVE To observe the effect of ginger moxibustion on vasoactive intestinal peptide(VIP) and interleukin-10(IL-10) in gastric cancer rats.METHODS The gastric carcinoma models， established by N-methyl-N-nitro-N-nitrosoguanidine(MNNG)， were randomly divided into 4 groups: VIP group(n=10， 10 μg/100 mL)，VIP antagonist group(n=10， 10 μg/100 mL)， model group(n=10， the normal breeding) and ginger moxibustion group(ginger moxibustion， n=10， on zhongwan， pishu， weishu， zusanli， neiguan， etc). Apart from that， blank group(n=10， physiological saline) was set up additionally. Three weeks later， the contents of VIP， IL-10 were tested， and the expression of proteins VIP and mRNA in gastric cancer tissues were detected by immunohistrochemistry and RT-PCR methods.RESULTS VIP and IL-10 in model group and were reduced， and the ones in ginger moxibustion group were increased obviously(P<0.01)， which have significant differences with the VIP group. Gastric carcinoma group have VIP-positive immunoreactive cells， which were not found in normal gastric tissues. There was VIP mRNA expression in each groups' gastric cancer tissue. The expression of mRNA in Ginger moxibustion and VIP groups were reduced， but ginger moxibustion group was more significantly reduced(P<0.01).CONCLUSION Gastric cancer rats can be treated with ginger moxibustion by inhibiting the detection of VIP， IL-10 and the expression of VIP mRNA. |
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