桔梗多糖对H2O2诱导PC12细胞氧化损伤的保护作用及机制研究

顾程远; 陈秋利; 李海涛*

文章摘要

顾程远，陈秋利，李海涛*.桔梗多糖对H2O2诱导PC12细胞氧化损伤的保护作用及机制研究[J].南京中医药大学学报(社会科学版),2017,33(3):268-272.

桔梗多糖对H2O2诱导PC12细胞氧化损伤的保护作用及机制研究

Protective Effects and Mechanism of Polysaccharide from Platycodon Grandiflorum on Damage of PC12 Cells Induced by H₂O₂

DOI：

中文关键词: 桔梗多糖抗氧化 PC12细胞 H₂O₂ NADPH氧化酶2

英文关键词: Platycodon grandiflorum polysaccharide antioxidation PC12 cells hydrogen peroxide NOX2

基金项目:

作者	单位
顾程远，陈秋利，李海涛*	南京中医药大学药学院，江苏南京 210023

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中文摘要:

目的研究桔梗多糖（PGP）对H2O2致PC12细胞氧化应激损伤的保护作用机制。方法建立H2O2诱导PC12细胞氧化损伤模型，MTT法检测细胞存活率；比色法测定细胞内乳酸脱氢酶（LDH）、丙二醛（MDA）含量、超氧化物歧化酶（SOD）以及谷胱甘肽过氧化物酶（GSH-Px）的活性；DCFH-DA检测细胞内ROS；qPCR、Western Blot方法分别检测NOX2、p22和Rac mRNA和蛋白的表达。结果与模型组相比，PGP预处理24h后，加入终浓度为600 μmol/L H2O2处理2h，LDH、MDA含量和细胞内ROS减少，SOD与GSH-Px活性增强，同时，PGP下调NOX2、p22和Rac mRNA和蛋白的表达水平。结论 PGP对H2O2诱导的PC12细胞的氧化损伤具有保护作用，其作用机制可能与抑制NADPH氧化酶2(NOX2)过表达有关。

英文摘要:

OBJECTIVE To determine the protective effects of Platycodon grandiflorum polysaccharide (PGP) on PC12 cells injured by H₂O₂ and the underlying mechanisms. METHODS The cell viability was analyzed by MTT assay， the model was established by treating PC12 cells with H₂O₂. The levels of LDH， MDA， SOD and GSH-Px were determined by Kits. ROS production was determined by DCFH-DA fluorescence. mRNA and proteins of NOX2， p22* and Rac were analyzed by qPCR and Western Blot methods. RESULTS Compared with the model， treatment with PGP significantly decrease of LDH， MDA and intracellular ROS production， as well as increase activity of SOD and GSH-Px. Furthermore， PGP could down-regulate mRNA and proteins of NOX2， p22* and Rac. CONCLUSION PGP exhibited protective effect on PC12 cells injury induced by H₂O₂， it may via the suppression of NOX2 activation.

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